SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N) (His tag)
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- Target See all SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) Proteins
- SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)
- Protein Type
- Recombinant
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Origin
- SARS Coronavirus-2 (SARS-CoV-2)
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This SARS-CoV-2 Nucleocapsid protein is labelled with His tag.
- Sequence
- AA 1-419
- Characteristics
- 2019-nCoV (COVID-19) Nucleocapsid protein, His Tag is expressed from E.coli cells. It contains AA Met 1 - Ala 419 (Accession # QHO62115.1). Predicted N-terminus: Met This protein carries a polyhistidine tag at the N-terminus.
- Purity
- >90 % as determined by SDS-PAGE.
- Endotoxin Level
- Less than 1.0 EU per μg by the LAL method.
- Top Product
- Discover our top product SARS-CoV-2 N Protein
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- Application Notes
- The protein has a calculated MW of 49.4 kDa. The protein migrates as 53-55 kDa under reducing (R) condition .
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 50 mM Tris, 150 mM NaCl, Arginine, pH 7.5
- Handling Advice
- Please avoid repeated freeze-thaw cycles.
- Storage
- -20 °C,-80 °C
- Storage Comment
- For long term storage, the product should be stored at lyophilized state at -20°C or lower. This product is stable aftr storage at: -20°C to -70°C for 12 months in lyophilized state, -70°C for 3 months under sterile conditions after reconstitution.
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- Target
- SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)
- Alternative Name
- SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N Products)
- Synonyms
- nucleocapsid phosphoprotein Protein, N Protein
- Target Type
- Viral Protein
- Background
- Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology, 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
- Molecular Weight
- 49.4 kDa
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