SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N) (His tag)

Catalog No. ABIN6952453

Product Details

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Protein Type: Recombinant

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This SARS-CoV-2 Nucleocapsid protein is labelled with His tag.

Sequence: AA 1-419

Characteristics: 2019-nCoV (COVID-19) Nucleocapsid protein, His Tag is expressed from E.coli cells. It contains AA Met 1 - Ala 419 (Accession # QHO62115.1). Predicted N-terminus: Met This protein carries a polyhistidine tag at the N-terminus.

Purity: >90 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Application Notes: The protein has a calculated MW of 49.4 kDa. The protein migrates as 53-55 kDa under reducing (R) condition .

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 50 mM Tris, 150 mM NaCl, Arginine, pH 7.5

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C,-80 °C

Storage Comment: For long term storage, the product should be stored at lyophilized state at -20°C or lower. This product is stable aftr storage at: -20°C to -70°C for 12 months in lyophilized state, -70°C for 3 months under sterile conditions after reconstitution.

Target Details

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Alternative Name: SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N Products)

Synonyms: nucleocapsid phosphoprotein Protein, N Protein

Target Type: Viral Protein

Background: Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology, 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.

Molecular Weight: 49.4 kDa