SARS-CoV-2 Spike Protein (SARS-CoV-2 S) (ECD) (His tag)

Catalog No. ABIN6952734

This Recombinant SARS-CoV-2 Spike protein is produced in HEK-293 Cells. There is 1 publication available.

Quick Overview for SARS-CoV-2 Spike Protein (SARS-CoV-2 S) (ECD) (His tag) (ABIN6952734)

Target: SARS-CoV-2 Spike (SARS-CoV-2 S)

Protein Type: Recombinant

Biological Activity: Active

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Application: SDS-PAGE (SDS), Functional Studies (Func)

Purity: >97 % by SDS-PAGE.

Product Details

Protein Characteristics: ECD

Purification tag / Conjugate: This SARS-CoV-2 Spike protein is labelled with His tag.

Sequence: VSSQCVNLTT RTQLPPAYTN SFTRGVYYPD KVFRSSVLHS TQDLFLPFFS NVTWFHAIHV SGTNGTKRFD NPVLPFNDGV YFASTEKSNI IRGWIFGTTL DSKTQSLLIV NNATNVVIKV CEFQFCNDPF LGVYYHKNNK SWMESEFRVY SSANNCTFEY VSQPFLMDLE GKQGNFKNLR EFVFKNIDGY FKIYSKHTPI NLVRDLPQGF SALEPLVDLP IGINITRFQT LLALHRSYLT PGDSSSGWTA GAAAYYVGYL QPRTFLLKYN ENGTITDAVD CALDPLSETK CTLKSFTVEK GIYQTSNFRV QPTESIVRFP NITNLCPFGE VFNATRFASV YAWNRKRISN CVADYSVLYN SASFSTFKCY GVSPTKLNDL CFTNVYADSF VIRGDEVRQI APGQTGKIAD YNYKLPDDFT GCVIAWNSNN LDSKVGGNYN YLYRLFRKSN LKPFERDIST EIYQAGSTPC NGVEGFNCYF PLQSYGFQPT NGVGYQPYRV VVLSFELLHA PATVCGPKKS TNLVKNKCVN FNFNGLTGTG VLTESNKKFL PFQQFGRDIA DTTDAVRDPQ TLEILDITPC SFGGVSVITP GTNTSNQVAV LYQDVNCTEV PVAIHADQLT PTWRVYSTGS NVFQTRAGCL IGAEHVNNSY ECDIPIGAGI CASYQTQTNS PRRARSVASQ SIIAYTMSLG AENSVAYSNN SIAIPTNFTI SVTTEILPVS MTKTSVDCTM YICGDSTECS NLLLQYGSFC TQLNRALTGI AVEQDKNTQE VFAQVKQIYK TPPIKDFGGF NFSQILPDPS KPSKRSFIED LLFNKVTLAD AGFIKQYGDC LGDIAARDLI CAQKFNGLTV LPPLLTDEMI AQYTSALLAG TITSGWTFGA GAALQIPFAM QMAYRFNGIG VTQNVLYENQ KLIANQFNSA IGKIQDSLSS TASALGKLQD VVNQNAQALN TLVKQLSSNF GAISSVLNDI LSRLDKVEAE VQIDRLITGR LQSLQTYVTQ QLIRAAEIRA SANLAATKMS ECVLGQSKRV DFCGKGYHLM SFPQSAPHGV VFLHVTYVPA QEKNFTTAPA ICHDGKAHFP REGVFVSNGT HWFVTQRNFY EPQIITTDNT FVSGNCDVVI GIVNNTVYDP LQPELDSFKE ELDKYFKNHT SPDVDLGDIS GINASVVNIQ KEIDRLNEVA KNLNESLIDL QELGKYEQ

Specificity: Val11-Gln1208

Characteristics: Recombinant 2019-nCoV S1+S2 ECD (S-ECD) Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val11-Gln1208) of 2019-ncov S1+S2 ECD (S-ECD) (Accession #YP_009724390.1) fused with a 6xHis tag at the C-terminus.

Sterility: 0.22 μm filtered

Endotoxin Level: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 Spike S1+S2 ECD-His at 2 μg/mL (100 μL/well) can bind Recombinant Human ACE2 with a linear range of 0.15-9.92 ng/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.95 mg/mL

Buffer: Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: This product is stable at ≤ -70°C for up to 6 months from the date of receipt. |For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.

References

Schlör, Hirschberg, Amor, Meister, Arora, Pöhlmann, Hoffmann, Pfaender, Eddin, Kamhieh-Milz, Hanack: "SARS-CoV-2 neutralizing camelid heavy-chain-only antibodies as powerful tools for diagnostic and therapeutic applications." in: Frontiers in immunology, Vol. 13, pp. 930975, (2022) (PubMed).

Target Details

Target: SARS-CoV-2 Spike (SARS-CoV-2 S)

Alternative Name: SARS-CoV-2 Spike

Target Type: Viral Protein

Background:

Description: The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates thisinteraction.The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity.

Name: Envelope,SARS-CoV-2 Spike RBD (N501Y),Spike,Spike ECD,Spike RBD,Spike S1,Spike S2,Spike S2 ECD,S1-RBD protein,NCP-CoV RBD Protein,novel coronavirus RBD Protein,2019-nCoV RBD Protein,S glycoprotein Subunit1 RBD Protein

Gene ID: 43740568

UniProt: P0DTC2