SARS-CoV-2 Spike Protein (Super Stable Trimer) (His tag)
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- Target See all SARS-CoV-2 Spike Proteins
- SARS-CoV-2 Spike
- Protein Type
- Recombinant
- Protein Characteristics
- Super Stable Trimer
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Origin
- SARS Coronavirus-2 (SARS-CoV-2)
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SARS-CoV-2 Spike protein is labelled with His tag.
- Application
- ELISA
- Purpose
- SARS-CoV-2 S protein, His Tag, Super stable trimer (MALS verified)
- Sequence
- AA 16-1213
- Characteristics
- SARS-CoV-2 S protein, His Tag, Super stable trimer is the ectodomain of SARS-CoV-2 S protein which contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
- Purity
- > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1.0 EU per μg by the LAL method.
- Top Product
- Discover our top product SARS-CoV-2 Spike Protein
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SARS-CoV-2 Spike (Super Stable Trimer) protein (His tag)
Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS. ELISA
SARS-CoV-2 Spike (RBD) protein (His-Avi Tag)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
SARS-CoV-2 Spike (N501Y), (RBD) protein (His tag)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike (E484K), (K417N), (N501Y), (RBD) protein (His tag)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike (B.1.617.2 - delta), (RBD) protein (Fc Tag)Origin: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Delta Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
SARS-CoV-2 Spike (B.1.1.7 - alpha), (RBD) protein (Fc Tag)Origin: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Alpha Host: HEK-293 Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining.
SARS-CoV-2 Spike (RBD) protein (mFc-His Tag)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining. ELISA, WB
SARS-CoV-2 Spike (D614G), (Trimer) protein (His-Avi Tag)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike (BA.2 - Omicron), (RBD) protein (His tag)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike (B.1.640), (RBD) protein (His tag)Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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- Application Notes
- This product is specifically designed for serological test development.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- PBS
- Storage
- -80 °C
- Storage Comment
- The product MUST be stored at -70°C or lower upon receipt.
- Expiry Date
- 3 months
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- Target
- SARS-CoV-2 Spike
- Abstract
- SARS-CoV-2 Spike Products
- Target Type
- Viral Protein
- Background
- It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
- Molecular Weight
- 138.0 kDa
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