CD276 Protein (CD276) (AA 29-245) (His tag,mFc Tag)

Catalog No. ABIN6961085

Recombinant CD276 protein expressed in HEK-293 Cells.

Quick Overview for CD276 Protein (CD276) (AA 29-245) (His tag,mFc Tag) (ABIN6961085)

Target: CD276

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 29-245

Purification tag / Conjugate: This CD276 protein is labelled with His tag,mFc Tag.

Purpose: Recombinant human B7-H3 protein with C-terminal mouse Fc and 6xHis tag

Specificity: B7-H3(Leu29-Pro245) mFc(Pro99-Lys330) 6xHis

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: CD276

Alternative Name: B7-H3

Background: B7-H3,CD276,B7 homolog 3
The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: predicted molecular mass of 70-75 kDa after removal of the signal peptide.

Gene ID: 80381

UniProt: Q5ZPR3

Pathways: Cancer Immune Checkpoints