Poliovirus Receptor Protein (PVR) (AA 21-343) (His tag,mFc Tag)

Catalog No. ABIN6961100

Recombinant Poliovirus Receptor protein expressed in HEK-293 Cells.

Quick Overview for Poliovirus Receptor Protein (PVR) (AA 21-343) (His tag,mFc Tag) (ABIN6961100)

Target: Poliovirus Receptor (PVR)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Screening Assay (ScA), Immunoassay (IA), Affinity Measurement (AM), Antibody Drug Assay (ADA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 21-343

Purification tag / Conjugate: This Poliovirus Receptor protein is labelled with His tag,mFc Tag.

Purpose: Recombinant human CD155 protein with C-terminal mouse Fc and 6xHis tag

Specificity: CD155(Trp21-Asn343) mFc(Pro99-Lys330) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: Poliovirus Receptor (PVR)

Alternative Name: CD155

Background: PVR,FLJ25946,PVS,CD155,TAGE4,HVED,NECL5
The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: predicted molecular mass of 62.2 kDa after removal of the signal peptide. The apparent molecular mass of CD155-mFc-His is 95-130 kDa due to glycosylation.

Gene ID: 5817

UniProt: P15151

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Cancer Immune Checkpoints, SARS-CoV-2 Protein Interactome