BTLA Protein (AA 31-150) (His tag,mFc Tag)

Catalog No. ABIN6961116

This Recombinant BTLA protein is produced in HEK-293 Cells.

Quick Overview for BTLA Protein (AA 31-150) (His tag,mFc Tag) (ABIN6961116)

Target: BTLA (B and T Lymphocyte Associated (BTLA))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 31-150

Purification tag / Conjugate: This BTLA protein is labelled with His tag,mFc Tag.

Purpose: Recombinant human BTLA protein with C-terminal mouse Fc and 6xHis tag

Specificity: BTLA(Lys31-Ser150) mFc(Pro99-Lys330) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: BTLA (B and T Lymphocyte Associated (BTLA))

Alternative Name: BTLA

Background: BTLA,CD272
This gene encodes a member of the immunoglobulin superfamily. The encoded protein contains a single immunoglobulin (Ig) domain and is a receptor that relays inhibitory signals to suppress the immune response. Alternative splicing results in multiple transcript variants. Polymorphisms in this gene have been associated with an increased risk of rheumatoid arthritis.

Molecular Weight: predicted molecular mass of 41.3 kDa after removal of the signal peptide.

Gene ID: 151888

UniProt: Q7Z6A9

Pathways: Cancer Immune Checkpoints