BTLA Protein (Fc Tag,ECD)

Catalog No. ABIN7321059

Product Details

Target: BTLA (B and T Lymphocyte Associated (BTLA))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: HEK-293 Cells

Purification tag / Conjugate: This BTLA protein is labelled with Fc Tag,ECD.

Purpose: Recombinant Rat BTLA/CD272 Protein (ECD, Fc Tag)(Active)

Sequence: Ala6-Tyr183

Characteristics: A DNA sequence encoding the rat Btla (NP_998795.1) (Ala6-Tyr183) was expressed with the Fc region of mouse IgG1 at the C-terminus.

Purity: > 90 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg protein as determined by the LAL method.

Biological Activity Comment: 1. Measured by its binding ability in a functional ELISA.2. Immobilized rat BTLA-mFc at 10 μg/mL (100 μL/well) can bind mouse HVEM-Fch . The EC50 of mouse EFNA5-Fc is 6.2-14.4 ng/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: BTLA (B and T Lymphocyte Associated (BTLA))

Alternative Name: BTLA/CD272 (BTLA Products)

Synonyms: BTLA Protein, BTLA1 Protein, CD272 Protein, A630002H24 Protein, B and T lymphocyte associated Protein, BTLA Protein, Btla Protein

Background:

Background: BTLA is a inhibitory molecule which belongs to the Ig superfamily. It down-modulates immune responses. As such, reagents that regulate the binding of BTLA to its ligand or alter BTLA signaling have significant therapeutic promise. BTLA is crucial to understand the mechanism(s) of action of these antibodies before attempting clinical applications. BTLA is not expressed by naive T cells, but it is induced during activation and remains expressed on T helper type 1 (T(H)1) but not T(H)2 cells. BTLA is a third inhibitory receptor on T lymphocytes with similarities to cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) and programmed death 1 (PD-1).Immune CheckpointImmune Checkpoint Targets   Co-inhibitory Immune Checkpoint Targets Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Synonym: BTLA

Molecular Weight: 43.4 kDa

NCBI Accession: NP_998795

Pathways: Cancer Immune Checkpoints