NCR3 Protein (AA 19-135) (Fc Tag)

Catalog No. ABIN6961135

This Recombinant NCR3 protein is expressed in HEK-293 Cells.

Quick Overview for NCR3 Protein (AA 19-135) (Fc Tag) (ABIN6961135)

Target: NCR3 (Natural Cytotoxicity Triggering Receptor 3 (NCR3))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 19-135

Purification tag / Conjugate: This NCR3 protein is labelled with Fc Tag.

Purpose: Recombinant human NKp30 protein with C-terminal human Fc

Specificity: NKp30(Leu19-Gly135) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: NCR3 (Natural Cytotoxicity Triggering Receptor 3 (NCR3))

Alternative Name: NKp30

Background: NCR3,CD337,NKp30,1C7,LY117,MALS
The protein encoded by this gene is a natural cytotoxicity receptor (NCR) that may aid NK cells in the lysis of tumor cells. The encoded protein interacts with CD3-zeta (CD247), a T-cell receptor. A single nucleotide polymorphism in the 5' untranslated region of this gene has been associated with mild malaria suceptibility. Three transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: predicted molecular mass of 39.0 kDa after removal of the signal peptide.The apparent molecular mass of NKp30-hFc is 45-60 kDa due to glycosylation.

Gene ID: 259197

UniProt: O14931

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process