IL-5 Protein (AA 20-134) (His tag)

Catalog No. ABIN6961138

This Recombinant IL-5 protein is expressed in HEK-293 Cells.

Quick Overview for IL-5 Protein (AA 20-134) (His tag) (ABIN6961138)

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Drug Assay (ADA), Affinity Measurement (AM), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 20-134

Purification tag / Conjugate: This IL-5 protein is labelled with His tag.

Purpose: Recombinant Human IL5 protein with C-terminal 6xHis tag

Specificity: IL5(Ile20-Ser134) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Alternative Name: IL5

Background: IL-5,TRF,Interleukin-5,EDF
This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.

Molecular Weight: predicted molecular mass of 13.94 kDa after removal of the signal peptide.The apparent molecular mass of IL5-His is 15-25 kDa due to glycosylation.

Gene ID: 3567

UniProt: P05113

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour