TIGIT Protein (AA 22-141) (mFc Tag)

Catalog No. ABIN6961182

This Recombinant TIGIT protein is produced in HEK-293 Cells.

Quick Overview for TIGIT Protein (AA 22-141) (mFc Tag) (ABIN6961182)

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 22-141

Purification tag / Conjugate: This TIGIT protein is labelled with mFc Tag.

Purpose: Recombinant human TIGIT Protein with C-terminal mouse Fc tag

Specificity: TIGIT(Met22-Pro141) mFc(Pro99-Lys330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Alternative Name: TIGIT

Background: TIGIT,VSIG9,VSTM3
This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity, this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses.

Molecular Weight: predicted molecular mass of 39.24 kDa after removal of the signal peptide.The apparent molecular mass of TIGIT-mFc is 40-53 kDa due to glycosylation.

Gene ID: 201633

UniProt: Q495A1

Pathways: Cancer Immune Checkpoints