TPBG Protein (AA 32-355) (His tag)

Catalog No. ABIN6964086

This Recombinant TPBG protein is produced in HEK-293 Cells.

Quick Overview for TPBG Protein (AA 32-355) (His tag) (ABIN6964086)

Target: TPBG (Trophoblast Glycoprotein (TPBG))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 32-355

Purification tag / Conjugate: This TPBG protein is labelled with His tag.

Purpose: Recombinant Human 5T4 Protein with C-terminal 6xHis tag

Specificity: 5T4(Ser32-Ser355) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: TPBG (Trophoblast Glycoprotein (TPBG))

Alternative Name: 5T4

Background: 5T4,5T4AG,M6P1,WAIF1
This gene encodes a leucine-rich transmembrane glycoprotein that may be involved in cell adhesion. The encoded protein is an oncofetal antigen that is specific to trophoblast cells. In adults this protein is highly expressed in many tumor cells and is associated with poor clinical outcome in numerous cancers. Alternate splicing in the 5' UTR results in multiple transcript variants that encode the same protein.

Molecular Weight: predicted molecular mass of 36.1 kDa after removal of the signal peptide.The apparent molecular mass of 5T4-His is 55-70 kDa due to glycosylation.

Gene ID: 7162

UniProt: Q13641