GPA33 Protein (AA 22-235) (Fc Tag)

Catalog No. ABIN6964123

This Recombinant GPA33 protein is expressed in HEK-293 Cells.

Quick Overview for GPA33 Protein (AA 22-235) (Fc Tag) (ABIN6964123)

Target: GPA33 (Glycoprotein A33 (Transmembrane) (GPA33))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 22-235

Purification tag / Conjugate: This GPA33 protein is labelled with Fc Tag.

Purpose: Recombinant Human GPA33 with C-terminal human Fc tag

Specificity: GPA33(Ile22-Val235) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: GPA33 (Glycoprotein A33 (Transmembrane) (GPA33))

Alternative Name: GPA33

Background: A33
The glycoprotein encoded by this gene is a cell surface antigen that is expressed in greater than 95 % of human colon cancers. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 2 domains characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily. [provided by RefSeq, Jul 2008]

Molecular Weight: predicted molecular mass of 49.8 kDa after removal of the signal peptide. The apparent molecular mass of GPA33-hFc is 55-70 kDa due to glycosylation.

UniProt: Q99795