CXCL1 Protein (AA 99-330) (Fc Tag)

Catalog No. ABIN6964250

Recombinant CXCL1 protein expressed in HEK-293 Cells.

Quick Overview for CXCL1 Protein (AA 99-330) (Fc Tag) (ABIN6964250)

Target: CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Immunoassay (IA), Affinity Measurement (AM), Antibody Drug Assay (ADA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 99-330

Purification tag / Conjugate: This CXCL1 protein is labelled with Fc Tag.

Purpose: Recombinant Human CXCL1 with N-terminal human Fc tag

Specificity: HFc(Glu99-Ala330) CXCL1(Ala35-Asn107)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))

Alternative Name: CXCL1

Background: FSP,GRO1,GROa,MGSA,MGSA-a,NAP-3,SCYB1
This antimicrobial gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. Aberrant expression of this protein is associated with the growth and progression of certain tumors. A naturally occurring processed form of this protein has increased chemotactic activity. Alternate splicing results in coding and non-coding variants of this gene. A pseudogene of this gene is found on chromosome 4. [provided by RefSeq, Sep 2014]

Molecular Weight: predicted molecular mass of 34.0 kDa after removal of the signal peptide.The apparent molecular mass of hFc-CXCL1 is 35-40 kDa due to glycosylation.

UniProt: P09341

Pathways: Autophagy