IFNAR2 Protein (AA 27-243) (Fc Tag)

Catalog No. ABIN6964274

Recombinant IFNAR2 protein expressed in HEK-293 Cells.

Quick Overview for IFNAR2 Protein (AA 27-243) (Fc Tag) (ABIN6964274)

Target: IFNAR2 (Interferon alpha/beta Receptor 2 (IFNAR2))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 27-243

Purification tag / Conjugate: This IFNAR2 protein is labelled with Fc Tag.

Purpose: Recombinant human IFNAR2 protein with C-terminal human Fc tag

Specificity: IFNAR2(Ile27-Lys243) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: IFNAR2 (Interferon alpha/beta Receptor 2 (IFNAR2))

Alternative Name: IFNAR2

Background: IFN-alpha-REC,IFN-R,IFNABR,IFNARB,IMD45
The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The protein belongs to the type II cytokine receptor family. Mutations in this gene are associated with Immunodeficiency 45. [provided by RefSeq, Jul 2020]

Molecular Weight: predicted molecular mass of 50.9 kDa after removal of the signal peptide. The apparent molecular mass of IFNAR2-hFc is 55-70 kDa due to glycosylation.

UniProt: P48551

Pathways: JAK-STAT Signaling, Hepatitis C