Prostate Specific Antigen Protein (PSA) (AA 18-261) (Fc Tag)

Catalog No. ABIN6964284

This Recombinant Prostate Specific Antigen protein is produced in HEK-293 Cells.

Quick Overview for Prostate Specific Antigen Protein (PSA) (AA 18-261) (Fc Tag) (ABIN6964284)

Target: Prostate Specific Antigen (PSA)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Immunoassay (IA), Screening Assay (ScA), Antibody Drug Assay (ADA), Affinity Measurement (AM)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 18-261

Purification tag / Conjugate: This Prostate Specific Antigen protein is labelled with Fc Tag.

Purpose: Recombinant human KLK3 Protein with C-terminal human Fc tag

Specificity: KLK3(Ala18-Pro261) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Purification: affinity purification

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: Prostate Specific Antigen (PSA)

Alternative Name: KLK3

Background: APS, PSA, hK3, KLK2A1
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. The gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. It encodes a single-chain glycoprotein, a protease which is synthesized in the epithelial cells of the prostate gland, and is present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. The serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Dec 2019]

Molecular Weight: predicted molecular mass of 53.0 kDa after removal of the signal peptide.

UniProt: P07288

Pathways: Complement System