GPC1 Protein (AA 24-530) (His tag)

Catalog No. ABIN6964386

This Recombinant GPC1 protein is produced in HEK-293 Cells.

Quick Overview for GPC1 Protein (AA 24-530) (His tag) (ABIN6964386)

Target: GPC1 (Glypican 1 (GPC1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 24-530

Purification tag / Conjugate: This GPC1 protein is labelled with His tag.

Purpose: Recombinant human GPC1 protein with C-terminal 7xHis tag

Specificity: GPC1(Asp24-Ser530) 7xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: GPC1 (Glypican 1 (GPC1))

Alternative Name: GPC1

Background: Glypican 1,GPC1
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq, Jul 2008]

Molecular Weight: predicted molecular mass of 57.0 kDa after removal of the signal peptide. The apparent molecular mass of GPC1-His is 55-70 kDa due to glycosylation.

UniProt: P35052

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation