NOS1 Protein (AA 726-894) (His tag)
Quick Overview for NOS1 Protein (AA 726-894) (His tag) (ABIN7404893)
Target
See all NOS1 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 726-894
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Purification tag / Conjugate
- This NOS1 protein is labelled with His tag.
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Purpose
- Recombinant Nitric Oxide Synthase 1, Neuronal (NOS1)
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Sequence
- Arg726-Asp894
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Characteristics
- Recombinant Nitric Oxide Synthase 1, Neuronal (NOS1), Prokaryotic expression, Arg726-Asp894 (Accession # P29476), N-terminal His Tag
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Endotoxin Level
- <1.0EU per 1μg (determined by the LAL method)
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Nitric Oxide Synthase 1, Neuronal (NOS1) (AA 101-301) protein (His tag)
NOS1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Nitric Oxide Synthase 1, Neuronal (NOS1) (AA 1041-1150) protein (GST tag)NOS1 Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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STABILITY: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Isoelectric Point: 9
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute in PBS or others.
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Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
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Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
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Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
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Expiry Date
- 12 months
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- NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))
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Alternative Name
- Nitric Oxide Synthase 1, Neuronal
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Background
- NOS, nNOS, IHPS1, NC-NOS, N-NOS, bNOS, Neuronal NOS, Constitutive NOS, NOS type I, Nitric Ocide Synthase, Peptidyl-cysteine S-nitrosylase NOS1
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Molecular Weight
- 16kDa
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UniProt
- P29476
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Pathways
- Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction
Target
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