PSMC3 Protein (AA 2-439) (His tag)
Quick Overview for PSMC3 Protein (AA 2-439) (His tag) (ABIN7414015)
Target
See all PSMC3 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 2-439
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Purification tag / Conjugate
- This PSMC3 protein is labelled with His tag.
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Purpose
- Recombinant Proteasome 26S Subunit, ATPase 3 (PSMC3)
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Sequence
- Asn2-Ala439
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Characteristics
- Recombinant Proteasome 26S Subunit, ATPase 3 (PSMC3), Prokaryotic expression, Asn2-Ala439, N-terminal His Tag
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Endotoxin Level
- <1.0EU per 1μg (determined by the LAL method)
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Proteasome (Prosome, Macropain) 26S Subunit, ATPase, 3 (PSMC3) (AA 1-439) protein (His tag)
custom-made PSMC3 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Proteasome (Prosome, Macropain) 26S Subunit, ATPase, 3 (PSMC3) (AA 1-442) protein (His tag)custom-made PSMC3 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Proteasome (Prosome, Macropain) 26S Subunit, ATPase, 3 (PSMC3) (AA 1-439) protein (GST tag)PSMC3 Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
Proteasome (Prosome, Macropain) 26S Subunit, ATPase, 3 (PSMC3) protein (Myc-DYKDDDDK Tag)PSMC3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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STABILITY: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Isoelectric Point: 5.6
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute in 10 mM PBS ( pH 7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
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Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
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Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
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Expiry Date
- 12 months
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- PSMC3 (Proteasome (Prosome, Macropain) 26S Subunit, ATPase, 3 (PSMC3))
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Alternative Name
- Proteasome 26S Subunit, ATPase 3
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Background
- TBP1, Tat-binding protein 1, Proteasome subunit P50, 26S proteasome AAA-ATPase subunit RPT5
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Molecular Weight
- 53kDa
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UniProt
- P17980
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Pathways
- Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Ubiquitin Proteasome Pathway
Target
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