RACGAP1 Protein (AA 106-539) (His tag,GST tag)

Catalog No. ABIN7397972

This Recombinant RACGAP1 protein is produced in Escherichia coli (E. coli).

Quick Overview for RACGAP1 Protein (AA 106-539) (His tag,GST tag) (ABIN7397972)

Target: RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), SDS-PAGE (SDS), Immunogen (Imm), Positive Control (PC)

Purity: > 80 %

Product Details

Protein Characteristics: AA 106-539

Purification tag / Conjugate: This RACGAP1 protein is labelled with His tag,GST tag.

Purpose: Recombinant Rac-GTPase Activating Protein 1 (RACGAP1)

Sequence: Met106-Trp539

Characteristics: Recombinant Rac-GTPase Activating Protein 1 (RACGAP1), Prokaryotic expression, Met106-Trp539, N-terminal His and GST Tag

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

STABILITY: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Isoelectric Point: 8.9

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in PBS or others.

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Expiry Date: 12 months

Target Details

Target: RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))

Alternative Name: Rac-GTPase Activating Protein 1

Background: HsCYK-4, ID-GAP, MgcRacGAP, Male germ cell RacGap

Molecular Weight: 78kDa

UniProt: Q9H0H5

Pathways: Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling of Hepatocyte Growth Factor Receptor