SMAD5 Protein (AA 210-464) (His tag)
Quick Overview for SMAD5 Protein (AA 210-464) (His tag) (ABIN7413646)
Target
See all SMAD5 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 210-464
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Purification tag / Conjugate
- This SMAD5 protein is labelled with His tag.
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Purpose
- Recombinant SMAD family member 5 (SMAD5)
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Sequence
- Gly210-Val464
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Characteristics
- Recombinant SMAD family member 5 (SMAD5), Prokaryotic expression, Gly210-Val464 (Accession # Q99717), N-terminal His Tag
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Endotoxin Level
- <1.0EU per 1μg (determined by the LAL method)
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SMAD, Mothers Against DPP Homolog 5 (SMAD5) (AA 186-465) protein (His-IF2DI Tag)
SMAD5 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
SMAD, Mothers Against DPP Homolog 5 (SMAD5) (AA 1-465) protein (GST tag)SMAD5 Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
SMAD, Mothers Against DPP Homolog 5 (SMAD5) (AA 2-465) protein (His-GST)SMAD5 Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
SMAD, Mothers Against DPP Homolog 5 (SMAD5) (AA 2-465) proteinSMAD5 Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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STABILITY: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Isoelectric Point: 6.4
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute in 20 mM Tris, 150 mM NaCl ( pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.
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Preservative
- Dithiothreitol (DTT), ProClin
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Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
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Expiry Date
- 12 months
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- SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))
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Alternative Name
- SMAD family member 5
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Background
- MADH5, Dwfc, JV5-1, Mothers Against Decapentaplegic Homolog 5
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Molecular Weight
- 32kDa
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UniProt
- Q99717
Target
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