UCP1 Protein (AA 179-296) (His tag)

Catalog No. ABIN7398515

This Recombinant UCP1 protein is produced in Escherichia coli (E. coli).

Quick Overview for UCP1 Protein (AA 179-296) (His tag) (ABIN7398515)

Target: UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Western Blotting (WB), Immunogen (Imm), Positive Control (PC)

Purity: > 97 %

Product Details

Protein Characteristics: AA 179-296

Purification tag / Conjugate: This UCP1 protein is labelled with His tag.

Purpose: Recombinant Uncoupling Protein 1, Mitochondrial (UCP1)

Sequence: Pro179-Leu296

Characteristics: Recombinant Uncoupling Protein 1, Mitochondrial (UCP1), Prokaryotic expression, Pro179-Leu296, N-terminal His Tag

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

STABILITY: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Isoelectric Point: 7.7

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 10 mM PBS ( pH 7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Expiry Date: 12 months

Target Details

Target: UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))

Alternative Name: Uncoupling Protein 1, Mitochondrial

Background: SLC25A7, UCP, Thermogenin, Solute carrier family 25 member 7

Molecular Weight: 13kDa

UniProt: P12242

Pathways: Proton Transport, Brown Fat Cell Differentiation