IL5RA Protein (AA 21-338) (Fc Tag)

Catalog No. ABIN7092703

This Recombinant IL5RA protein is produced in HEK-293 Cells.

Quick Overview for IL5RA Protein (AA 21-338) (Fc Tag) (ABIN7092703)

Target: IL5RA (Interleukin 5 Receptor, alpha (IL5RA))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 21-338

Purification tag / Conjugate: This IL5RA protein is labelled with Fc Tag.

Purpose: Recombinant human L5RA protein with C-terminal human Fc tag

Specificity: IL5RA(Asp21-Pro338) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: IL5RA (Interleukin 5 Receptor, alpha (IL5RA))

Alternative Name: IL5RA

Background: IL5Ra,CD125,IL-5 R alpha
The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Several alternatively spliced transcript variants encoding four distinct isoforms have been reported.

Molecular Weight: predicted molecular mass of 62.3 kDa after removal of the signal peptide.The apparent molecular mass of IL5RA-hFc is 70-100 kDa due to glycosylation.

Gene ID: 3567

UniProt: Q01344

Pathways: JAK-STAT Signaling