Complement Factor B Protein (CFB) (AA 26-764) (Fc Tag)

Catalog No. ABIN7092743

This Recombinant Complement Factor B protein is expressed in HEK-293 Cells.

Quick Overview for Complement Factor B Protein (CFB) (AA 26-764) (Fc Tag) (ABIN7092743)

Target: Complement Factor B (CFB)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 26-764

Purification tag / Conjugate: This Complement Factor B protein is labelled with Fc Tag.

Purpose: Recombinant Human CFB with C-terminal human Fc tag

Specificity: CFB(Thr26-Leu764) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: Complement Factor B (CFB)

Alternative Name: CFB

Background: AHUS4,ARMD14,BF,BFD,CFAB,CFBD,FB,FBI12,GBG,H2-Bf,PBF2
This gene encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. Polymorphisms in this gene are associated with a reduced risk of age-related macular degeneration. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2. [provided by RefSeq, Jul 2008]

Molecular Weight: predicted molecular mass of 109.2 kDa after removal of the signal peptide.The apparent molecular mass of CFB-hFc is 100-130 kDa due to glycosylation.

UniProt: P00751

Pathways: Complement System, Proton Transport, Ribonucleoside Biosynthetic Process