Complement Factor B Protein (CFB) (AA 26-764) (His tag)

Catalog No. ABIN1096421

Product Details

Target: Complement Factor B (CFB)

Protein Type: Recombinant

Protein Characteristics: AA 26-764

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This Complement Factor B protein is labelled with His tag.

Purpose: Recombinant Human Complement Factor B/CFB (C-6His)

Sequence: TPWSLAWPQG SCSLEGVEIK GGSFRLLQEG QALEYVCPSG FYPYPVQTRT CRSTGSWSTL KTQDQKTVRK AECRAIHCPR PHDFENGEYW PRSPYYNVSD EISFHCYDGY TLRGSANRTC QVNGRWSGQT AICDNGAGYC SNPGIPIGTR KVGSQYRLED SVTYHCSRGL TLRGSQRRTC QEGGSWSGTE PSCQDSFMYD TPQEVAEAFL SSLTETIEGV DAEDGHGPGE QQKRKIVLDP SGSMNIYLVL DGSDSIGASN FTGAKKCLVN LIEKVASYGV KPRYGLVTYA TYPKIWVKVS EADSSNADWV TKQLNEINYE DHKLKSGTNT KKALQAVYSM MSWPDDVPPE GWNRTRHVII LMTDGLHNMG GDPITVIDEI RDLLYIGKDR KNPREDYLDV YVFGVGPLVN QVNINALASK KDNEQHVFKV KDMENLEDVF YQMIDESQSL SLCGMVWEHR KGTDYHKQPW QAKISVIRPS KGHESCMGAV VSEYFVLTAA HCFTVDDKEH SIKVSVGGEK RDLEIEVVLF HPNYNINGKK EAGIPEFYDY DVALIKLKNK LKYGQTIRPI CLPCTEGTTR ALRLPPTTTC QQQKEELLPA QDIKALFVSE EEKKLTRKEV YIKNGDKKGS CERDAQYAPG YDKVKDISEV VTPRFLCTGG VSPYADPNTC RGDSGGPLIV HKRSRFIQVG VISWGVVDVC KNQKRQKQVP AHARDFHINL FQVLPWLKEK LQDEDLGFLV DHHHHHH

Characteristics: Recombinant Human Complement Factor B/CFB is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Thr26-Leu764) of Human CFB fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: Complement Factor B (CFB)

Alternative Name: complement-factor-b (CFB Products)

Synonyms: AHUS4 Protein, BF Protein, BFD Protein, CFAB Protein, FB Protein, FBI12 Protein, GBG Protein, H2-Bf Protein, PBF2 Protein, bf Protein, Bf-1 Protein, Brre-Bf Protein, zgc:114032 Protein, Bf Protein, Sp152 Protein, bfb-A Protein, Bf B Protein, LOC397725 Protein, bf/c2 Protein, CFB Protein, AI195813 Protein, AI255840 Protein, B Protein, C2 Protein, Fb Protein, Da1-24 Protein, complement factor B Protein, complement factor B S homeolog Protein, complement factor B L homeolog Protein, CFB Protein, cfb Protein, bf Protein, cfb.S Protein, cfb.L Protein, bf/c2 Protein, LOC100555981 Protein, Cfb Protein

Sub Type: Fusionprotein

Background: Complement Factor B (CFB) belongs to the peptidase S1 family of enzymes. It is expressed by hepatocytes and macrophages and localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. CFB which is a component of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.
Alternative Names: Complement Factor B, C3/C5 Convertase, Glycine-Rich Beta Glycoprotein, GBG, PBF2, Properdin Factor B, CFB, BF, BFD

Molecular Weight: 84.07 kDa

UniProt: P00751

Pathways: Complement System, Proton Transport, Ribonucleoside Biosynthetic Process