ANTXR1 Protein (AA 33-321) (His tag)

Catalog No. ABIN7092772

This Recombinant ANTXR1 protein is produced in HEK-293 Cells.

Quick Overview for ANTXR1 Protein (AA 33-321) (His tag) (ABIN7092772)

Target: ANTXR1 (anthrax Toxin Receptor 1 (ANTXR1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 33-321

Purification tag / Conjugate: This ANTXR1 protein is labelled with His tag.

Purpose: Recombinant human ANTXR1 protein with C-terminal 6xHis tag

Specificity: ANTXR1(Glu33-Ser321) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: ANTXR1 (anthrax Toxin Receptor 1 (ANTXR1))

Alternative Name: ANTXR1

Background: ATR,GAPO,TEM8
This gene encodes a type I transmembrane protein and is a tumor-specific endothelial marker that has been implicated in colorectal cancer. The encoded protein has been shown to also be a docking protein or receptor for Bacillus anthracis toxin, the causative agent of the disease, anthrax. The binding of the protective antigen (PA) component, of the tripartite anthrax toxin, to this receptor protein mediates delivery of toxin components to the cytosol of cells. Once inside the cell, the other two components of anthrax toxin, edema factor (EF) and lethal factor (LF) disrupt normal cellular processes. Three alternatively spliced variants that encode different protein isoforms have been described. [provided by RefSeq, Oct 2008]

Molecular Weight: predicted molecular mass of 33.2 kDa after removal of the signal peptide. The apparent molecular mass of ANTXR1-His is 35-55 kDa due to glycosylation.

UniProt: Q9H6X2