FZD10 Protein (AA 21-161) (Fc Tag)

Catalog No. ABIN7092805

This Recombinant FZD10 protein is produced in HEK-293 Cells.

Quick Overview for FZD10 Protein (AA 21-161) (Fc Tag) (ABIN7092805)

Target: FZD10 (Frizzled Family Receptor 10 (FZD10))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 21-161

Purification tag / Conjugate: This FZD10 protein is labelled with Fc Tag.

Purpose: Recombinant Human FZD10 with C-terminal human Fc tag

Specificity: FZD10(Ile21-Gly161) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: FZD10 (Frizzled Family Receptor 10 (FZD10))

Alternative Name: FZD10

Background: CD350,FZ-10,Fz10,FzE7,hFz10
This gene is a member of the frizzled gene family. Members of this family encode 7-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. Using array analysis, expression of this intronless gene is significantly up-regulated in two cases of primary colon cancer. [provided by RefSeq, Jul 2008]

Molecular Weight: predicted molecular mass of 42.2 kDa after removal of the signal peptide. The apparent molecular mass of FZD10-hFc is 35-55 kDa due to glycosylation.

UniProt: Q9ULW2

Pathways: WNT Signaling