DR3/LARD Protein (AA 31-196) (His tag)

Catalog No. ABIN7274495

Recombinant DR3/LARD protein expressed in HEK-293 Cells.

Quick Overview for DR3/LARD Protein (AA 31-196) (His tag) (ABIN7274495)

Target: DR3/LARD (TNFRSF25) (Tumor Necrosis Factor Receptor Superfamily, Member 25 (TNFRSF25))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 31-196

Purification tag / Conjugate: This DR3/LARD protein is labelled with His tag.

Purpose: Mouse DR3/TNFRSF25 Protein

Sequence: Gln31-Met196

Characteristics: Recombinant Mouse DR3/TNFRSF25 Protein is expressed from HEK293 with His tag at the C-Terminus., It contains Gln31-Met196.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse TNFSF15, hFc Tag captured on CM5 Chip via Protein A can bind Mouse DR3, His Tag with an affinity constant of 4.56 nM as determined in SPR assay (Biacore T200).,Immobilized Mouse DR3, His Tag at 1ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Mouse TNFSF15, His Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test).,Mouse DR3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Mouse TNFSF15, monomeric Fc Tag on ProA-Biosensor, can bind Mouse DR3, His Tag with an affinity constant of 22.80 nM as determined in BLI assay (Gator).,The purity of Mouse DR3 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: DR3/LARD (TNFRSF25) (Tumor Necrosis Factor Receptor Superfamily, Member 25 (TNFRSF25))

Alternative Name: DR3

Background: Death Receptor 3 (DR3), also known as TNFRSF25, TRAMP, LARD, or WSL-1, is a death-domain-containing TNF-family receptor that, like its closest paralog TNFR1, binds the adaptor molecule TRADD through its cytoplasmic death domain. TRADD recruitment endows DR3 with dual-signaling capability to activate NF-κB and MAP-kinase signaling or alternatively trigger caspase activation and programmed cell death.

Molecular Weight: 18.79 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Bis-Tris PAGE result.

Pathways: Apoptosis