Gastrin-Releasing Peptide Protein (GRP) (AA 24-50) (Fc Tag)

Catalog No. ABIN7092808

This Recombinant Gastrin-Releasing Peptide protein is produced in HEK-293 Cells.

Quick Overview for Gastrin-Releasing Peptide Protein (GRP) (AA 24-50) (Fc Tag) (ABIN7092808)

Target: Gastrin-Releasing Peptide (GRP)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 24-50

Purification tag / Conjugate: This Gastrin-Releasing Peptide protein is labelled with Fc Tag.

Purpose: Recombinant Human GRP with C-terminal human Fc tag

Specificity: GRP(Val24-Met50) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: Gastrin-Releasing Peptide (GRP)

Alternative Name: GRP

Background: BN,GRP-10,preproGRP,proGRP
This gene encodes a member of the bombesin-like family of gastrin-releasing peptides. The encoded preproprotein is proteolytically processed to generate two peptides, gastrin-releasing peptide and neuromedin-C. These peptides regulate numerous functions of the gastrointestinal and central nervous systems, including release of gastrointestinal hormones, smooth muscle cell contraction, and epithelial cell proliferation. These peptides are also likely to play a role in human cancers of the lung, colon, stomach, pancreas, breast, and prostate. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]

Molecular Weight: predicted molecular mass of 29.0 kDa after removal of the signal peptide. The apparent molecular mass of GRP-hFc is 25-35 kDa due to glycosylation.

UniProt: P07492

Pathways: Peptide Hormone Metabolism, Hormone Activity