IGFBP7 Protein (AA 30-282) (His tag)

Catalog No. ABIN7092833

This Recombinant IGFBP7 protein is expressed in HEK-293 Cells.

Quick Overview for IGFBP7 Protein (AA 30-282) (His tag) (ABIN7092833)

Target: IGFBP7 (Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 30-282

Purification tag / Conjugate: This IGFBP7 protein is labelled with His tag.

Purpose: Recombinant human IGFBP7 protein with N-terminal 6xHis tag

Specificity: 6xHis tag IGFBP7(Asp30-Leu282)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: IGFBP7 (Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))

Alternative Name: IGFBP7

Background: AGM,FSTL2,IBP-7,IGFBP-7,IGFBP-7v,IGFBPRP1,MAC25,PSF,RAMSVPS,TAF
This gene encodes a member of the insulin-like growth factor (IGF)-binding protein (IGFBP) family. IGFBPs bind IGFs with high affinity, and regulate IGF availability in body fluids and tissues and modulate IGF binding to its receptors. This protein binds IGF-I and IGF-II with relatively low affinity, and belongs to a subfamily of low-affinity IGFBPs. It also stimulates prostacyclin production and cell adhesion. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and one variant has been associated with retinal arterial macroaneurysm (PMID:21835307). [provided by RefSeq, Dec 2011]

Molecular Weight: predicted molecular mass of 27.0 kDa after removal of the signal peptide. The apparent molecular mass of His-IGFBP7 is 35-40 kDa due to glycosylation.

UniProt: Q16270

Pathways: Growth Factor Binding