LRP5 Protein (His-Avi Tag,Biotin)

Catalog No. ABIN7121511

This Recombinant LRP5 protein is expressed in HEK-293 Cells.

Quick Overview for LRP5 Protein (His-Avi Tag,Biotin) (ABIN7121511)

Target: LRP5 (Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: >95 % as determined by SDS-PAGE.

Product Details

Purification tag / Conjugate: This LRP5 protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Human LRP-5 Protein, His,Avitag™

Sequence: Glu 644 - Gln 1263

Characteristics: Biotinylated Human LRP-5, His,Avitag (LR5-H82E6) is expressed from human 293 cells (HEK293). It contains AA Glu 644 - Gln 1263 (Accession # O75197-1 ).

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Application Notes: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 73.3 kDa. The protein migrates as 70-90 kDa under reducing (R) condition due to glycosylation.

Comment:

Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Storage: -20 °C

Storage Comment: -20°C

Target Details

Target: LRP5 (Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))

Alternative Name: LRP-5

Background: Synonyms: LRP5,LRP-5,LRP-7,LRP7,LR3,
Description: Low-density lipoprotein receptor-related protein 5(LRP-5) is also known as BMND1, EVR1, EVR4, HBM, LR3, LRP7, OPPG, OPTA1, VBCH2, LDL receptor related protein 5 and PCLD4. LRP5 is a transmembrane low-density lipoprotein receptor that shares a similar structure with LRP6. LRP5 acts as a co-receptor with LRP6 and the Frizzled protein family members for transducing signals by Wnt proteins through the canonical Wnt pathway. This protein plays a key role in skeletal homeostasis. Mutations in LRP5 can lead to considerable changes in bone mass. A loss-of-function mutation causes osteoporosis-pseudoglioma (decrease in bone mass), while a gain-of-function mutation causes drastic increases in bone mass.

Molecular Weight: 73.3 kDa

NCBI Accession: NP_002326

Pathways: WNT Signaling, Stem Cell Maintenance, Positive Regulation of fat Cell Differentiation