ATG5 Protein (AA 138-275) (His-IF2DI Tag)

Catalog No. ABIN7121921

This Recombinant ATG5 protein is produced in Escherichia coli (E. coli).

Quick Overview for ATG5 Protein (AA 138-275) (His-IF2DI Tag) (ABIN7121921)

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: ELISA, Western Blotting (WB)

Purity: Greater than 90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 138-275

Purification tag / Conjugate: This ATG5 protein is labelled with His-IF2DI Tag.

Purpose: Recombinant Human ATG5

Sterility: 0.2 μm filtered

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Expiry Date: 12 months

Target Details

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Alternative Name: ATG5

Background: APG5 autophagy 5-like (S. cerevisiae), APG5, APG5LS. cerevisiae)-like, ASP, ASPAPG5-LIKE, ATG5 autophagy related 5 homolog (S. cerevisiae), ATG5, Autophagy protein 5

Molecular Weight: 36.9 kDa

UniProt: Q9H1Y0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy