ATG5 Protein
Quick Overview for ATG5 Protein (ABIN7318187)
Target
See all ATG5 ProteinsProtein Type
Origin
Source
Purity
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Purpose
- Recombinant Human ATG5 Protein
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Sequence
- Met 1-Asp275
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Characteristics
- Recombinant Human Autophagy Related 5 Homolog is produced by our E.coli expression system and the target gene encoding Met1-Asp275 is expressed.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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ATG5 Autophagy Related 5 (ATG5) (AA 1-275) protein (His tag)
custom-made ATG5 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
ATG5 Autophagy Related 5 (ATG5) (AA 1-275) protein (His tag)custom-made ATG5 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
ATG5 Autophagy Related 5 (ATG5) (AA 1-275) protein (GST tag)ATG5 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
ATG5 Autophagy Related 5 (ATG5) (AA 138-275) protein (His-IF2DI Tag)ATG5 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
ATG5 Autophagy Related 5 (ATG5) (AA 1-275) protein (His-IF2DI Tag)ATG5 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 0.2M NaCl, 1 mM DTT, 40 % Glycerol, pH 8.0.
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Preservative
- Dithiothreitol (DTT)
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Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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Expiry Date
- 6 months
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- ATG5 (ATG5 Autophagy Related 5 (ATG5))
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Alternative Name
- ATG5
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Background
- APG5-like,APG5L,ASP,ATG5,Apoptosis-specific protein,Autophagy protein 5,ATG5 is an E2 ubiquitin ligase which is necessary for autophagy. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity, dramatically highly expressed in apoptotic cells. It is activated by ATG7, conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate complex detaches from the membrane immediately before or after autophagosome formation is completed. ATG5 plays an important role in the apoptotic process, possibly within the modified cytoskeleton.
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Molecular Weight
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Calculated MW: 32.4 kDa
Observed MW: 33 kDa
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Gene ID
- 9474
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UniProt
- Q9H1Y0
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Pathways
- Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy
Target
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