Caspase 9 Protein (AA 1-159) (His tag)

Catalog No. ABIN7122171

This Recombinant Caspase 9 protein is expressed in Escherichia coli (E. coli).

Quick Overview for Caspase 9 Protein (AA 1-159) (His tag) (ABIN7122171)

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), ELISA

Purity: Greater than 90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 1-159

Purification tag / Conjugate: This Caspase 9 protein is labelled with His tag.

Purpose: Recombinant Human CASP9

Sterility: 0.2 μm filtered

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Expiry Date: 12 months

Target Details

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Alternative Name: CASP9

Background: APAF-3APAF3, apoptotic protease activating factor 3, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP9, CASP-9, caspase 9, apoptosis-related cysteine peptidase, Caspase9, Caspase-9, EC 3.4.22.62, ICE-LAP6CASPASE-9c

Molecular Weight: 19.8 kDa

UniProt: P55211

Pathways: MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Endopeptidase Activity