EIF4E Protein (AA 1-217) (His tag)

Catalog No. ABIN7122850

Recombinant EIF4E protein expressed in Escherichia coli (E. coli).

Quick Overview for EIF4E Protein (AA 1-217) (His tag) (ABIN7122850)

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: ELISA, Western Blotting (WB)

Purity: Greater than 90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 1-217

Purification tag / Conjugate: This EIF4E protein is labelled with His tag.

Purpose: Recombinant Mouse eIF4E

Sterility: 0.2 μm filtered

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Expiry Date: 12 months

Target Details

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Alternative Name: eIF4E

Background: AUTS19, CBP, eIF 4E, EIF 4F, eIF 4F 25 kDa subunit, eIF-4E, eIF-4F 25 kDa subunit, eIF4E, EIF4E1, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4 E, Eukaryotic translation initiation factor 4E, Eukaryotic translation initiation factor 4E like 1

Molecular Weight: 26.9 kDa

UniProt: P63073

Pathways: BCR Signaling