EIF4E Protein (AA 1-217) (His tag)

Catalog No. ABIN6387994

Product Details

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Protein Type: Recombinant

Protein Characteristics: AA 1-217

Origin: Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This EIF4E protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMATVEP ETTPTTNPPP AEEEKTESNQ EVANPEHYIK HPLQNRWALW FFKNDKSKTW QANLRLISKF DTVEDFWALY NHIQLSSNLM PGCDYSLFKD GIEPMWEDEK NKRGGRWLIT LNKQQRRSDL DRFWLETLLC LIGESFDDYS DDVCGAVVNV RAKGDKIAIW TTECENRDAV THIGRVYKER LGLPPKIVIG YQSHADTATK SGSTTKNRFV V

Purity: > 95 % by SDS - PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In 20 mM Tris-HCL Buffer (  pH 8.0) containing 10 % glycerol, 1  mM DTT, 0.1M NaCl, 0.1  mM PMSF

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Alternative Name: Eif4e (EIF4E Products)

Synonyms: AUTS19 Protein, CBP Protein, EIF4E1 Protein, EIF4EL1 Protein, EIF4F Protein, eif4e-1 Protein, zgc:86680 Protein, EG668879 Protein, Eif4e-ps Protein, If4e Protein, eIF-4E Protein, 0260/09 Protein, 0587/11 Protein, 0589/11 Protein, 0919/12 Protein, 1004/13 Protein, 2 Protein, 7238 Protein, CG4035 Protein, CT13384 Protein, CT39424 Protein, CT39426 Protein, D-eIF4E Protein, Dmel\\CG4035 Protein, Eif4E Protein, Eif4e Protein, IF4E Protein, dEif4e Protein, deIF-4E Protein, deIF4E Protein, eIF 4E Protein, eIF-4E1 Protein, eIF-4E2 Protein, eIF-4EII Protein, eIF-4e Protein, eIF4E Protein, eIF4E-1 Protein, eIF4E-1/2 Protein, eIF4E-2 Protein, eIF4E1 Protein, eIF4EI Protein, eIF4e Protein, eif-4E Protein, eif-4e Protein, eif4e Protein, l(3)07238 Protein, l(3)67Af Protein, l(3)S025007 Protein, l(3)S026009 Protein, l(3)S058711 Protein, l(3)S091912 Protein, cbp Protein, eif4f Protein, eif4e1 Protein, eif4el1 Protein, EIF-4E Protein, zgc:101581 Protein, eukaryotic translation initiation factor 4E Protein, eukaryotic translation initiation factor 4ea Protein, Eukaryotic initiation factor 4E Protein, eucaryotic initiation factor 6 Protein, eukaryotic translation initiation factor 4E L homeolog Protein, eukaryotic translation initiation factor 4E family member 1B S homeolog Protein, translation initiation factor eIF4E Protein, eukaryotic translation initiation factor 4eb Protein, EIF4E Protein, eif4ea Protein, Eif4e Protein, eIF-4E Protein, eif6 Protein, eif4e Protein, eif4e.L Protein, eif4e1b.S Protein, LOC100533326 Protein, eif4eb Protein

Background: Eif4e, also known as Eukaryotic translation initiation factor 4E, modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. This protein recognizes and binds to the 7 methyl GTP cap structure of eukaryotic mRNAs, thereby mediating the initiation of translation. It facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Recombinant mouse Eif4e protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Molecular Weight: 27.6kDa (241aa) confirmed by MALDI-TOF

NCBI Accession: NP_031943

UniProt: P63073

Pathways: BCR Signaling