IL1A Protein (AA 115-270) (His tag)

Catalog No. ABIN7123511

This Recombinant IL1A protein is produced in Escherichia coli (E. coli).

Quick Overview for IL1A Protein (AA 115-270) (His tag) (ABIN7123511)

Target: IL1A (Interleukin 1 alpha (IL1A))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), ELISA

Purity: Greater than 90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 115-270

Purification tag / Conjugate: This IL1A protein is labelled with His tag.

Purpose: Recombinant Mouse IL-1A

Sterility: 0.2 μm filtered

Endotoxin Level: < 1 EU/μg as determined by LAL test.

Biological Activity Comment: Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells.The ED50 for this effect is 10-50 pg/mL.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Expiry Date: 12 months

Target Details

Target: IL1A (Interleukin 1 alpha (IL1A))

Alternative Name: IL-1A

Background: BAF, FAF, Hematopoietin 1, Hematopoietin-1, IL 1 alpha, IL 1A, IL-1 alpha, Il-1a, IL1, IL1 ALPHA, IL1A, IL1A_HUMAN, IL1F1, Interleukin 1 alpha

Molecular Weight: 17.1 kDa

UniProt: P01582

Pathways: NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints