LIF Protein (AA 24-203) (His tag)

Catalog No. ABIN7123758

This Recombinant LIF protein is expressed in Escherichia coli (E. coli).

Quick Overview for LIF Protein (AA 24-203) (His tag) (ABIN7123758)

Target: LIF (Leukemia Inhibitory Factor (LIF))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), ELISA

Purity: Greater than 95 % as determined by SDS-PAGE

Product Details

Protein Characteristics: AA 24-203

Purification tag / Conjugate: This LIF protein is labelled with His tag.

Sterility: 0.2 μm filtered

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/mL in sterile distilled water by gentle pipetting 2-3 times, don't vortex

Buffer: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4

Storage: 4 °C,-20 °C

Storage Comment: -20°C for 12 months as lyophilized, 2-8°C for 1 month under sterile conditions after reconstitution

Expiry Date: 12 months

Target Details

Target: LIF (Leukemia Inhibitory Factor (LIF))

Alternative Name: LIF

Background: CDF, Cholinergic Differentiation Factor, D factor, DIA, Differentiation inducing factor, differentiation inhibitory activity, Differentiation stimulating factor, Differentiation-stimulating factor, Emfilermin, Hepatocyte stimulating factor III, HILDA, Human interleukin in DA cells

Molecular Weight: 19.7 kDa

UniProt: P09056

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding