LEF1 Protein (AA 120-399) (His tag)
This Recombinant LEF1 protein is expressed in Escherichia coli (E. coli).
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Catalog No. ABIN7123803
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Quick Overview for LEF1 Protein (AA 120-399) (His tag) (ABIN7123803)
Target
See all LEF1 Proteins
LEF1
(Lymphoid Enhancer-Binding Factor 1 (LEF1))
Protein Type
Recombinant
Origin
Human
Source
Escherichia coli (E. coli)
Application
ELISA, Western Blotting (WB)
Purity
Greater than 90 % as determined by SDS-PAGE.
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Protein Characteristics
- AA 120-399
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Purification tag / Conjugate
- This LEF1 protein is labelled with His tag.
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Purpose
- Recombinant Human LEF1
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Sterility
- 0.2 μm filtered
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Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 1-399) protein (GST tag)
LEF1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.
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Buffer
- Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.
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Storage
- -20 °C,-80 °C
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Storage Comment
- The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.
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Expiry Date
- 12 months
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- LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))
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Alternative Name
- LEF1
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Background
- DKFZp586H0919, FLJ46390, LEF 1, LEF-1, Lef1, LEF1_HUMAN, Lymphoid enhancer binding factor 1, Lymphoid enhancer-binding factor 1, T cell specific transcription factor 1 alpha, T cell-specific transcription factor 1-alpha, TCF 1 alpha, TCF1 alpha, TCF1-alpha, TCF10, TCF1alpha
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Molecular Weight
- 33.3 kDa
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UniProt
- Q9UJU2
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Pathways
- WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding
Target
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