MBD4 Protein (AA 68-300) (His-IF2DI Tag)

Catalog No. ABIN7123938

This Recombinant MBD4 protein is expressed in Escherichia coli (E. coli).

Quick Overview for MBD4 Protein (AA 68-300) (His-IF2DI Tag) (ABIN7123938)

Target: MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: ELISA, Western Blotting (WB)

Purity: Greater than 90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 68-300

Purification tag / Conjugate: This MBD4 protein is labelled with His-IF2DI Tag.

Purpose: Recombinant Human MBD4

Sterility: 0.2 μm filtered

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Expiry Date: 12 months

Target Details

Target: MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))

Alternative Name: MBD4

Background: 3 N(4) ethenocytosine glycosylase, G/5 fluorouracil mismatch glycosylase with biphasic kinetics, G/T mismatch glycosylase, G/U mismatch glycosylase, MBD 4, MBD4, MBD4_HUMAN, MED 1, MED1, Methyl CpG binding domain protein 4, Methyl CpG binding endonuclease 1, Methyl CpG binding protein MBD4

Molecular Weight: 25.5 kDa

UniProt: O95243

Pathways: DNA Damage Repair