PDGFB Protein (AA 82-190) (His tag)

Catalog No. ABIN7124479

Recombinant PDGFB protein expressed in Escherichia coli (E. coli).

Quick Overview for PDGFB Protein (AA 82-190) (His tag) (ABIN7124479)

Target: PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), ELISA

Purity: Greater than 90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 82-190

Purification tag / Conjugate: This PDGFB protein is labelled with His tag.

Purpose: Recombinant Human PDGFB

Sterility: 0.2 μm filtered

Biological Activity Comment: Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells.The ED50 for this effect is 2-10 ng/mL.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Expiry Date: 12 months

Target Details

Target: PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))

Alternative Name: PDGFB

Background: PDGFBB, PDGF-BB

Molecular Weight: 14.1 kDa

UniProt: P01127

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling