PKLR Protein (AA 47-574) (His tag)
Quick Overview for PKLR Protein (AA 47-574) (His tag) (ABIN7124725)
Target
See all PKLR ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 47-574
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Purification tag / Conjugate
- This PKLR protein is labelled with His tag.
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Purpose
- Recombinant Human PKLR
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Sterility
- 0.2 μm filtered
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Pyruvate Kinase, Liver and RBC (PKLR) (AA 1-574) protein (His tag)
custom-made PKLR Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Pyruvate Kinase, Liver and RBC (PKLR) (AA 1-574) protein (His tag)custom-made PKLR Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Pyruvate Kinase, Liver and RBC (PKLR) (AA 1-574) protein (GST tag)PKLR Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Pyruvate Kinase, Liver and RBC (PKLR) (AA 20-105) (Active) protein (hFc Tag)PKLR Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
Pyruvate Kinase, Liver and RBC (PKLR) (AA 47-574) (Active) protein (His tag)PKLR Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS, EAA Active
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.
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Buffer
- Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.
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Storage
- -20 °C,-80 °C
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Storage Comment
- The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.
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Expiry Date
- 12 months
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- PKLR (Pyruvate Kinase, Liver and RBC (PKLR))
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Alternative Name
- PKLR
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Background
- EC 2.7.1.40, KPYR_HUMAN, L-PK, Pk-1, PK1, PKL, Pklg, Pklr, PKR, PKRL, Pyruvate kinase 1, Pyruvate kinase isozymes R/L, Pyruvate kinase liver and blood cell, Pyruvate kinase liver and RBC, Pyruvate kinase liver and red blood cell, Pyruvate kinase liver type
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Molecular Weight
- 58 kDa
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UniProt
- P30613
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Pathways
- Ribonucleoside Biosynthetic Process
Target
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