RHOA Protein (AA 61-193) (His-IF2DI Tag)
Quick Overview for RHOA Protein (AA 61-193) (His-IF2DI Tag) (ABIN7124783)
Target
See all RHOA ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 61-193
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Purification tag / Conjugate
- This RHOA protein is labelled with His-IF2DI Tag.
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Purpose
- Recombinant Human RHOA
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Sterility
- 0.2 μm filtered
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Ras Homolog Gene Family, Member A (RHOA) (AA 1-193) protein (GST tag)
RHOA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Ras Homolog Gene Family, Member A (RHOA) (AA 1-193) protein (His-IF2DI Tag)RHOA Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Ras Homolog Gene Family, Member A (RHOA) protein (Myc-DYKDDDDK Tag)RHOA Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Ras Homolog Gene Family, Member A (RHOA) (AA 1-191), (partial) protein (GST tag)RHOA Origin: Human Host: Escherichia coli (E. coli) Recombinant 95 % WB, SDS, ELISA
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.
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Buffer
- Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.
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Storage
- -20 °C,-80 °C
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Storage Comment
- The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.
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Expiry Date
- 12 months
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- RHOA (Ras Homolog Gene Family, Member A (RHOA))
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Alternative Name
- RHOA
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Background
- Aplysia ras related homolog 12, ARH12, ARHA, H 12, H12, Oncogene RHO H12, Ras homolog family member A, Ras homolog gene family member A, Rho A, Rho cDNA clone 12, RHO H12, RHO12, RHOA, RHOA_HUMAN, RHOH12, Small GTP binding protein Rho A, Transforming protein Rho A, Transforming protein RhoA
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Molecular Weight
- 35 kDa
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UniProt
- P61586
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Pathways
- Microtubule Dynamics, WNT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, SARS-CoV-2 Protein Interactome
Target
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