POLR2J Protein (AA 1-117) (His-IF2DI Tag)

Catalog No. ABIN7124929

Recombinant POLR2J protein expressed in Escherichia coli (E. coli).

Quick Overview for POLR2J Protein (AA 1-117) (His-IF2DI Tag) (ABIN7124929)

Target: POLR2J (Polymerase (RNA) II (DNA Directed) Polypeptide J, 13.3kDa (POLR2J))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: ELISA, Western Blotting (WB)

Purity: Greater than 90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 1-117

Purification tag / Conjugate: This POLR2J protein is labelled with His-IF2DI Tag.

Purpose: Recombinant Human POLR2J

Sterility: 0.2 μm filtered

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Expiry Date: 12 months

Target Details

Target: POLR2J (Polymerase (RNA) II (DNA Directed) Polypeptide J, 13.3kDa (POLR2J))

Alternative Name: POLR2J

Background: DNA directed RNA polymerase II subunit RPB11 a, DNA-directed RNA polymerase II 13.3 kDa polypeptide, DNA-directed RNA polymerase II subunit J 1, DNA-directed RNA polymerase II subunit J-1, DNA-directed RNA polymerase II subunit RPB11-a, hRPB 14, hRPB14, POLR 2J1

Molecular Weight: 12.8 kDa

UniProt: P52435

Pathways: Regulatory RNA Pathways