POLR2J Protein (Myc-DYKDDDDK Tag)
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- Target See all POLR2J Proteins
- POLR2J (Polymerase (RNA) II (DNA Directed) Polypeptide J, 13.3kDa (POLR2J))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This POLR2J protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human POLR2J protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product POLR2J Protein
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Polymerase (RNA) II (DNA Directed) Polypeptide J, 13.3kDa (POLR2J) (AA 1-117) protein (GST tag)
POLR2J Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Polymerase (RNA) II (DNA Directed) Polypeptide J, 13.3kDa (POLR2J) (AA 1-117) protein (Strep Tag)Crystallography grade POLR2J Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Polymerase (RNA) II (DNA Directed) Polypeptide J, 13.3kDa (POLR2J) (AA 1-117) protein (Strep Tag)Crystallography grade POLR2J Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- POLR2J (Polymerase (RNA) II (DNA Directed) Polypeptide J, 13.3kDa (POLR2J))
- Alternative Name
- Polr2j (POLR2J Products)
- Synonyms
- POLR2J1 Protein, RPB11 Protein, RPB11A Protein, RPB11m Protein, hRPB14 Protein, POLR2J2 Protein, 14.5kDa Protein, Polr2i Protein, Rpb11a Protein, Rpo2-4 Protein, RNA polymerase II subunit J Protein, polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa S homeolog Protein, polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa Protein, polymerase (RNA) II (DNA directed) polypeptide J Protein, POLR2J Protein, Polr2j Protein, polr2j.S Protein, polr2j Protein
- Background
- This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13.
- Molecular Weight
- 13.1 kDa
- NCBI Accession
- NP_006225
- Pathways
- Regulatory RNA Pathways
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