ELAPOR1 Protein (His-Avi Tag,Biotin)

Catalog No. ABIN7199653

This Recombinant ELAPOR1 protein is expressed in HEK-293 Cells.

Quick Overview for ELAPOR1 Protein (His-Avi Tag,Biotin) (ABIN7199653)

Target: ELAPOR1 (Endosome/Lysosome-associated Apoptosis and Autophagy Regulator 1 (ELAPOR1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: >95 % as determined by SDS-PAGE.

Product Details

Purification tag / Conjugate: This ELAPOR1 protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Human ELAPOR1 Protein, His,Avitag™

Sequence: Thr 42 - Lys 910

Characteristics: Biotinylated Human ELAPOR1, His,Avitag is expressed from human 293 cells (HEK293). It contains AA Thr 42 - Lys 910 (Accession # Q6UXG2-1).

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Application Notes: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 99.0 kDa. The protein migrates as kDa under reducing (R) condition due to glycosylation.

Comment:

Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Storage: -20 °C

Storage Comment: For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Target Details

Target: ELAPOR1 (Endosome/Lysosome-associated Apoptosis and Autophagy Regulator 1 (ELAPOR1))

Alternative Name: ELAPOR1

Background: Synonyms: ELAPOR1,Endosome-Lysosome Associated Apoptosis And Autophagy Regulator 1,EIG121,KIAA1324,
Description: Endosome/lysosome-associated apoptosis and autophagy regulator (ELAPOR1), also known as EIG121 protein, is a type I transmembrane protein induced by estrogen. It is associated with the endosome-lysosome compartments and may play an important role in autophagy and cell proliferation. Under unfavorable conditions such as starvation and exposure to cytotoxic agents, ELAPOR1 may protect cells from cell death by upregulating the autophagy pathway.

Molecular Weight: 99.0 kDa