beta Actin Protein (His tag)
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- Target See all beta Actin (ACTB) Proteins
- beta Actin (ACTB) (Actin, beta (ACTB))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This beta Actin protein is labelled with His tag.
- Purpose
- Recombinant Human β-Actin/Beta Actin Protein (His Tag)
- Sequence
- Asp2-Phe375
- Characteristics
- Recombinant Human beta-Actin is produced by our E.coli expression system and the target gene encoding Asp2-Phe375 is expressed with a 6His tag at the C-terminus.
- Purity
- > 85 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product ACTB Protein
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Actin, beta (ACTB) (AA 1-375) protein (His tag)
custom-made ACTB Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Actin, beta (ACTB) (AA 1-375) protein (His tag)custom-made ACTB Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Actin, beta (ACTB) (AA 1-375) protein (GST tag)ACTB Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Actin, beta (ACTB) (AA 14-167) protein (His tag)ACTB Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Actin, beta (ACTB) (AA 102-375) protein (His tag)ACTB Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
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- Restrictions
- For Research Use only
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- Format
- Frozen, Liquid
- Buffer
- Supplied as a 0.2 μm filtered solution of 10 mM TrisHCl, 0.1 % TritonX-100, 2 mM DTT, pH 8.0.
- Storage
- -20 °C
- Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- Target
- beta Actin (ACTB) (Actin, beta (ACTB))
- Alternative Name
- beta-Actin/Beta Actin (ACTB Products)
- Background
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Background: Actins are ubiquitous globular and highly conserved proteins that are involved in various types of cell motility, structure, and integrity. Three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. ACTB is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.
Synonym: Actin Cytoplasmic 1, Beta-Actin, ACTB
- Molecular Weight
- 42.8 kDa
- UniProt
- P60709
- Pathways
- Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
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