AK4 Protein (His-GST)

Catalog No. ABIN7194160

This Recombinant AK4 protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for AK4 Protein (His-GST) (ABIN7194160)

Target: AK4 (Adenylate Kinase 4 (AK4))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 94 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This AK4 protein is labelled with His-GST.

Purpose: Recombinant Human AK4/AK3L1 Protein (His & GST Tag)

Sequence: Ala 2-Tyr 223

Characteristics: A DNA sequence encoding the human AK4 (P27144-1) (Ala 2-Tyr 223) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 8.0

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: AK4 (Adenylate Kinase 4 (AK4))

Alternative Name: AK4/AK3L1

Background: AK3,AK3L1,AK3L2,AK4,Adenylate kinase isoenzyme 4, mitochondrial, also known as ATP-AMP transphosphorylase, Adenylate kinase 3-like, AK4 and AK3L1, is a member theadenylate kinase family. AK4 / AK3L1 is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. AK4 / AK3L1 catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. It may also be active with GTP. Adenylate kinase 4 ( AK4 / AK3L1 ) is a unique member with no enzymatic activity in the adenylate kinase (AK) family although it shares high sequence homology with other AKs. It remains unclear what physiological function AK4 might play or why it is enzymatically inactive. AK4 / AK3L1 retains the capability of binding nucleotides. It has a glutamine residue instead of a key arginine residue in the active site well conserved in other AKs. The enzymatically inactive AK4 is a stress responsive protein critical to cell survival and proliferation. AK4 / AK3L1 is likely that the interaction with the mitochondrial inner membrane protein ANT is important for AK4 to exert the protective benefits to cells under stress. AK4 / AK3L1 also acts on the specific mechanism of energy metabolism rather than control of the homeostasis of the ADP pool ubiquitously.

Molecular Weight:

Calculated MW: 53.0 kDa

Observed MW: 46 kDa

Gene ID: 205

UniProt: P27144

Pathways: Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process