Adenylosuccinate Lyase Protein (ADSL) (His tag)

Catalog No. ABIN7317594

This Recombinant Adenylosuccinate Lyase protein is produced in Escherichia coli (E. coli).

Quick Overview for Adenylosuccinate Lyase Protein (ADSL) (His tag) (ABIN7317594)

Target: Adenylosuccinate Lyase (ADSL)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This Adenylosuccinate Lyase protein is labelled with His tag.

Purpose: Recombinant Human ADSL/Adenylosuccinate Lyase Protein (His Tag)

Sequence: Met 1-Leu 484

Characteristics: A DNA sequence encoding the human ADSL (P30566-1) (Met 1-Leu 484) was expressed, with a polyhistidine tag at the N-terminus.

Sterility: 0.2 μm filtered

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, 10 % glycerol, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Adenylosuccinate Lyase (ADSL)

Alternative Name: ADSL/Adenylosuccinate Lyase

Background: AMPS,ASASE,ASL,Adenylosuccinate lyase, also known as adenylosuccinase, ADSL or ASL, is an enzyme implicated in the reaction of adenylosuccinat converting to AMP and fumarate as part of the purine nucleotide cycle. The two substates of adenylosuccinate lyase (ADSL) are dephosphorylated derivatives of SAICA ribotide (SAICAR) and adenylosuccinate (S-AMP), which catalyzes an important reaction in the de novo pathway of purine biosynthesis. ADSL catalyzes two distinct reactions in the synthesis of purine nucleotides, both of which involve the _-elimination of fumarate to produce either aminoimidazole carboxamide ribotide from SAICAR or AMP from S-AMP. The Adenylosuccinate lyase deficiency is a rare autosomal recessive metabolic disorder characterized by the present of SAICA riboside and succinyladenosine (S-Ado). ADSL defect in different patients is often caused by different mutations to the enzyme.

Molecular Weight:

Calculated MW: 57.0 kDa

Observed MW: 53 kDa

Gene ID: 158

UniProt: P30566

Pathways: Ribonucleoside Biosynthetic Process