Fetuin A Protein (His tag)

Catalog No. ABIN7320410

Recombinant Fetuin A protein expressed in HEK-293 Cells.

Quick Overview for Fetuin A Protein (His tag) (ABIN7320410)

Target: Fetuin A (AHSG) (alpha-2-HS-Glycoprotein (AHSG))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This Fetuin A protein is labelled with His tag.

Purpose: Recombinant Mouse Fetuin-A/AHSG Protein (His Tag)(Active)

Sequence: Met1-Ile 345

Characteristics: A DNA sequence encoding the mouse AHSG (NP_032037.2) precursor (Met1-Ile 345) was expressed with a polyhistidine tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: 1. Measured by its binding ability in a functional ELISA. Immobilized human FCN1 at 10 μg/mL can bind biotinylated recombinant mouse Fetuin-A with a linear range of 16-2000 ng/ml.2. Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2, AnaSpec, Catalog # 27114. The IC50 value is <200 nM.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 25 mM Tris, 0.15 mM NaCl, pH 7.5

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Fetuin A (AHSG) (alpha-2-HS-Glycoprotein (AHSG))

Alternative Name: Fetuin-A/AHSG

Background:

Background: Fetuin-A, also known as Alpha-2-HS-Glycoprotein (AHSG), belongs to the Fetuin family, is a plasma binding protein, and is more abundant in fetal than adult blood. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. Fetuins are carrier proteins like albumin. Fetuin-A forms soluble complexes with calcium and phosphate and thus is a carrier of otherwise insoluble calcium phosphate. Thus Fetuin-A is a potent inhibitor of pathological calcification. The circulating levels of fetuin-A, a well-described inhibitor of calcification, regulate the cell-dependent process of osteogenesis. The low circulating fetuin-A levels are associated with a greater prevalence and/or severity of Vascular calcification (VC) and increased risk for all-cause and cardiovascular mortality. However, high circulating fetuin-A levels appear to induce insulin resistance and, in non-dialyzed subjects with diabetic nephropathy, are directly related to VC burden. The emerging role of fetuin-A deficiency as a risk factor in dialysis patients was documented in cross-sectional studies demonstrating a significant correlation with all-cause and cardiovascular mortality. Additionally, Human fetuin-A is a negative acute phase protein involved in inflammatory diseases, thus being a potential physiological regulator of meprin activity. Fetuin-A is a broad-range protease inhibitor. Fetuin-A and cystatin C as endogenous proteolytic regulators of meprin activity broadens our understanding of the proteolytic network in plasma.

Synonym: Alpha-2-HS-glycoprotein, Ahsg, Countertrypin, Fetuin-A, Fetua

Molecular Weight: 36.7 kDa

NCBI Accession: NP_032037