Fetuin A Protein (AA 19-345) (His tag)

Catalog No. ABIN7320686

This Recombinant Fetuin A protein is produced in HEK-293 Cells.

Quick Overview for Fetuin A Protein (AA 19-345) (His tag) (ABIN7320686)

Target: Fetuin A (AHSG) (alpha-2-HS-Glycoprotein (AHSG))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 19-345

Purification tag / Conjugate: This Fetuin A protein is labelled with His tag.

Purpose: Recombinant Mouse Fetuin-A/AHSG Protein (His Tag)

Sequence: Ala19-Ile345

Characteristics: Recombinant Mouse alpha-2-HS-Glycoprotein is produced by our Mammalian expression system and the target gene encoding Ala19-Ile345 is expressed with a 10His tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Fetuin A (AHSG) (alpha-2-HS-Glycoprotein (AHSG))

Alternative Name: Fetuin-A/AHSG

Background: Ahsg,Alpha-2-HS-glycoprotein,Countertrypin,Fetua,Fetuin-A,Alpha-2-HS-glycoprotein (AHSG) is a glycoprotein that is composed of two subunits, the A and B chains, belongs to the Cystatin family of proteases inhibitors. It is highly expressed in embryonic cells and adult hepatocytes, and is expressed to a lesser extent in monocytes/macrophages. AHSG is an important circulating inhibitor of calcification in vivo, and is downregulated during the acute-phase response. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. In addition, AHSG may influence the resolution of inflammation by modulating the phagocytosis of apoptotic cells by macrophages. ASHG blocks TGF-beta-dependent signaling in osteoblastic cells.

Molecular Weight:

Calculated MW: 36.7 kDa

Observed MW: 45-75 kDa

UniProt: P29699